Closed-system solid surface vitrification versus slow programmable freezing of mouse 2-cell embryos
نویسندگان
چکیده
منابع مشابه
Slow freezing, vitrification and ultra-rapid freezing of human embryos: a systematic review and meta-analysis.
Embryo cryopreservation is an important aspect of assisted reproduction. Many methods have been described, but they have been poorly investigated in randomized trials, highlighting the need for a systematic review of the literature. Meticulous electronic/hand searches were performed to locate randomized trials (RCT) comparing embryo cryopreservation methods. Primary outcomes were clinical pregn...
متن کاملIn vitro development and gene expression of frozen-thawed 8-cell stage mouse embryos following slow freezing or vitrification
OBJECTIVE This study was performed to compare the efficiency of slow freezing and vitrification based on survival, development to blastocysts, and cell numbers of blastocysts. Changes in embryonic gene expression in fresh and frozen-thawed embryos were also examined. METHODS Eight-cell stage embryos were collected from superovulated female BDF1 mice. The collected embryos were randomly divide...
متن کاملP-30: Developmental Capacity and Blastocyst Formation of Thawed Tetrahedral Versus Non-Tetrahedral 4-cell Stage Mouse Embryos After Vitrification
Background: It was reported in a literature that approximately one third of the 4-cell stage embryos did not exhibit a tetrahedral shape. Non-tetrahedral embryos showed a lower in vitro developmental potential than tetrahedral embryos. Recently vitrification technology has been widely employed for embryo cryopreservation. The objective of this study was to prove our hypothesis that vitrified - ...
متن کاملRepeated vitrification/warming of human sperm gives better results than repeated slow programmable freezing.
In this study, we compared the effects of repeated freezing/thawing of human sperm by our in-house method of rapid freezing with slow programmable freezing. Sperm samples from 11 normozoospermic subjects were processed through density gradients and divided into three aliquots: non-frozen, rapid freezing and slow programmable freezing. Sperm in the rapid freezing group had better motility and vi...
متن کاملEfficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device
BACKGROUND Currently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse embryos to determine whether this novel device can be adapted to the field of ART. METHODS In Experiment...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2009
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-009-9324-8